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• 產(chǎn)品描述： Zotarolimus, an analogue of rapamycin, inhibits FKBP-12 (IC50= 2.8 nM).

• 靶點(diǎn)： mTOR

• 體內(nèi)研究：
  Zotarolimus potently inhibits adjuvant DTH(ED50=1.72 mg/kg/day), EAE(ED50=1.17 mg/kg/day), and cardiac allograft rejection(ED50=3.71 mg/kg/day)

• 細(xì)胞實(shí)驗(yàn)： Cell proliferation is assayed by measuring tritiated thymidine incorporation in vitro. Human coronary artery cells (hCa) are seeded into tissue culture flasks for expansion and applied to 96-well plates at desired density in complete media (5000 hCaSMC; 10 000 hCaEC). After 2 days, complete media is replaced with incomplete media to synchronize cells and induce G0 state. Two days later, incomplete media are removed and replaced with complete media (serum/growth factors) to induce G0 to G1 transition. Complete media also contain drug at desired concentrations to determine its effects on cell proliferation. On day 7, 3H-thymidine is added to cells to monitor DNA synthesis, and cells are harvested after overnight incorporation of radioactivity. After an incubation period of 72 h, 25 μL (1 μCi/well) of 3H-thymidine are added to each well. The cells are incubated at 37°C for 16-18 h to allow for incorporation of 3H-thymidine into newly synthesized DNA and the cells harvested onto 96-well plates containing bonded g

• 動(dòng)物實(shí)驗(yàn)： Male Sprague-Dawley rats was administrate by intravenous or oral Zotarolimus（2.5 mg/kg）dissolved inethanol: propylene glycol: cremophor EL: D5W vehicle (20: 30: 2: 48, by volume).

• 參考文獻(xiàn)：
  1.Chen YW, et al. J Cardiovasc Pharmacol, 2007, 49(4), 228-235. 2.Garcia-Touchard A, et al. Eur Heart J, 2006, 27(8), 988-1993.

• 溶解性： Ethanol:93  mg/mL  (96.3  mM)    H2O:<1  mg/mL    DMSO:93  mg/mL  (96.3  mM)

• 保存條件： 2-8℃

• 配置溶液濃度參考：
  

  	1mg	5mg	10mg
  1 mM	1.035 ml	5.175 ml	10.35 ml
  5 mM	0.207 ml	1.035 ml	2.07 ml
  10 mM	0.103 ml	0.517 ml	1.035 ml
  50 mM	0.021 ml	0.103 ml	0.207 ml

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

輸入產(chǎn)品批號(hào)：

本計(jì)算器可幫助您計(jì)算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *